Journal: Molecular cell

Article Title: Differential scaling of gene expression with cell size may explain size control in budding yeast

doi: 10.1016/j.molcel.2020.03.012

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The total protein concentration in the WCE was determined by Bradford 1x dye reagent (BioRad).

Techniques: Recombinant, Fluorescence, Protease Inhibitor, Software

Journal: ACS Chemical Biology

Article Title: Hsp70 Inhibits the Nucleation and Elongation of Tau and Sequesters Tau Aggregates with High Affinity

doi: 10.1021/acschembio.7b01039

Figure Lengend Snippet: Hsp70 inhibits tau aggregation in a substoichiometric manner. (a) The extent of heparin induced fibrillization of K18 ΔK280 tau (10 μM), monitored by Thioflavin-T (ThT) fluorescence over the course of the aggregation reaction. N = 3, error bars are s. e. m. (b) Sedimentation assay: soluble and insoluble K18 ΔK280 tau formed after 24 h at different concentrations of Hsp70. Representative SDS-PAGE analysis, coomassie staining. N = 3. (c) Relative protein levels in supernatant and pellet by densitometry of SDS-PAGE bands. N = 3, error bars are s. e. m. (d) The aggregates obtained at the end of the aggregation reactions visualized on a TIRF microscope (pFTAA staining) and by TEM. Representative images ( N = 3). Scale bars: TIRFM 10 μm, TEM 500 nm.

Article Snippet: The aggregation was started by heparin addition and continued for the indicated amounts of time at 37° C. To analyze the amount of soluble and insoluble protein after given periods of time, each mixture was centrifuged at 16 000 g for 20 min. Supernatant and pellet fractions were then subjected to SDS-PAGE using 4–12% Bis-Tris gels (Bolt, Thermo Fisher Scientific) and protein bands stained with colloidal coomassie (BioRad).

Techniques: Fluorescence, Sedimentation, SDS Page, Staining, Microscopy